Journal: Journal of Applied Microbiology

Article Title: Comparative survival of environmental and clinical Mycobacterium abscessus isolates in a variety of diverse host cells

doi: 10.1111/jam.15416

Figure Lengend Snippet: Co‐culture of the isolate panel with healthy human epithelial cells or macrophages and cytokine/chemokine quantification. (a) TNFα quantification of BEAS‐2B culture supernatants 1 or 24 h post culture. ** p = 0.0041. (b) TNFα quantification of alveolar macrophage culture supernatants 1 and 24 h post culture. * p = 0.0112. For CFU, at least 3 or more independent experiments were performed for all isolates and for each cell type. For ELISA, n = 2 was performed for each cell type. These co‐culture experiments were performed at 37°C. Multiple analyte analyses of THP‐1 culture supernatants. Culture supernatants from THP‐1 co‐culture experiments after 24, 48 and 96 h detailed in this figure were collected and filtered through a 0.2 µm filter. IL‐8, IL‐10, IL‐6, IL‐1β and TNFα were quantified on the Quanterix Simoa Technology platform. 24‐h results are shown in (c), 48‐h results are shown in (d), and 96 h results are shown in (e). n = 3 independent experiments per timepoint. Values are represented as the pg/ml cytokine level divided by the THP‐1 uninfected cell supernatant pg/ml value at that timepoint. Median values are shown. Isolate 4 = Arthrobacter sps

Article Snippet: THP‐1 cell culture supernatants were filtered through a 0.2 µm filter for cytokine/chemokine analyses on the Quanterix Simoa Multiplex platform for TNFα, IL‐6, IL‐8, IL‐10 and IL‐1β (Children's Hospital, Colorado).

Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay